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Irre (im Singular) steht für: Orientierungsverlust oder Ziellosigkeit, siehe Auf dem Holzweg sein. Irre (im Plural) steht für: Geisteskranke (umgangssprachlich). Definition, Rechtschreibung, Synonyme und Grammatik von 'irre' auf Duden online nachschlagen. Wörterbuch der deutschen Sprache. ir·re, Komparativ: ir·rer, Superlativ: am irrs·ten. Aussprache: IPA: [ˈɪʁə]: Hörbeispiele: Lautsprecherbild irre: Reime. Irre, die oder der. Grammatik Substantiv (Femininum, Maskulinum) · Genitiv Singular: Irren · Nominativ Plural: Irre(n). irre (Deutsch). Wortart: Adjektiv. Steigerungen: Positiv irre, Komparativ irrer, Superlativ am irrsten. Nebenformen: irr. Silbentrennung.

irre

Synonyme für "irre" ▷ gefundene Synonyme ✓ 33 verschiedene Bedeutungen für irre ✓ Ähnliches & anderes Wort für irre. Many translated example sentences containing "irre" – English-German dictionary and search engine for English translations. Irre, die oder der. Grammatik Substantiv (Femininum, Maskulinum) · Genitiv Singular: Irren · Nominativ Plural: Irre(n).

Irre Video

Verdunkeln - Der Herrscher

We demonstrated here that expression of IrrE, a global regulator for extreme radiation resistance in Deinococcus radiodurans , confers significantly enhanced salt tolerance in both E.

Our data suggest that IrrE acts as a switch to control a set of genes involved in different metabolic and signaling pathways, leading to dramatically enhance salt tolerance in these genetically modified organisms.

To study the effect of IrrE in E. The effect of salt stress on the growth of control strain and IrrE-expressing strain was examined in M9 minimal medium.

When the IrrE-expressing strain was inoculated into M9 minimal medium, it also displayed better growth than the control strain with higher maximal cell density Figure 1B.

In the presence of 0. A: Growth of the E. To assay the effect of IrrE expression on salt tolerance in a crop plant, we generated Brassica napus transgenic plants overexpressing the irrE gene Figure 2.

As shown in Figure 2A , a construct containing the full-length cDNA of the irrE gene under the control of the cauliflower mosaic virus 35S promoter was introduced into the genome of Brassica napus cultivar Shuanzha no.

Following two rounds of kanamycin selection, kanamycin resistant plants were regenerated. PCR analysis revealed that 42 plants showed amplification of the predicted irrE gene fragment.

Seven homozygous lines from these transgenic plants were obtained in the T2 generation. Southern blot analysis suggested that seven transgenic lines had one or more copies of the irrE gene data not shown.

Western blot analysis confirmed the expression of IrrE in four independent transgenic lines, but not in the wild-type control plants Figure 2B.

The expression levels of IrrE vary among the different lines, which could be attributed to different integration sites of the transgene or different transgene copy number [20].

Molecular mass is indicated on the right; C Salt treatment of Wt1 and representative transgenic plants. After mM NaCl treatment for six weeks, representative plants were photographed.

Two weeks after germination, four independent transgenic lines of B. All transgenic plants grew well in th. Transgenic plants treated with mM NaCl were able to flower and produce seeds normally when transferred into the field.

No significant difference was detected between salt-treated transgenic plants and control plants that had not been exposed to salt stress, as evaluated by various agronomic traits such as plant height, length of main inflorescence, number of pods per plant, number of seeds per pod and weight of 1, seeds data not shown.

We further performed experiments to investigate the underlying molecular mechanisms of the enhanced global stress responses in IrrE-expressing E.

We first used two-dimensional protein gels to compare global changes in the proteome of the IrrE-expressing E. Both strains were subjected to high salt shock and the proteins were extracted and subjected to two-dimensional gel electrophoresis analysis Figure 3.

Using a cutoff criterion of 2. These up- or downregulated proteins can be grouped into 13 classes based on their common functional characteristics, indicating that IrrE plays a global regulatory role in gene expression in E.

Among these 13 classes, five major groups might contribute in various ways to the enhanced salt resistance: i Stress responsive proteins.

RpoS is known to be a fundamental regulator of general stress responses to various stress conditions in E.

We found significant induction of RpoS, which exhibits a 3-fold-higher expression in the IrrE-expressing strain than in the control strain.

Furthermore, accumulation of a set of proteins usually induced by environmental stress was observed in the IrrE-expressing strain, including the molecular chaperone DnaK, the heat shock protein HslU and the osmotically inducible protein OsmY Figure 3.

In addition, about twenty other proteins not detectable in the control strain were induced in the IrrE-expressing strain Table S1 , including the general stress protein YhbO required for the protection of bacterial cells against many environmental stresses [23] , the trigger factor Tig involved in protein export as a chaperone, and the stress-inducible ATP-dependent protease Lon which is induced under stress conditions such as high temperatures [16].

In microorganisms and plants, protein kinases generally act as sensory molecules in response to environmental changes. We found nine protein kinases that were up- or downregulated with a ratio exceeding two in the IrrE - expressing strain compared to the control strain.

Expression of nucleoside diphosphate kinase Ndk, which is responsible for maintaining a pool of nucleotide triphosphates for the synthesis of DNA and RNA in E.

None of the levels of enzymes involved in glycerol biosynthesis showed obvious changes. The respiratory metabolism of glycerol in E.

We examined glycerol levels in an IrrE-expressing strain and a control strain subjected to salt treatment. Accumulation of high levels of glycerol, a major feature of osmoregulation in fungi [27] , could lead to improved tolerance to various abiotic stresses; iv Detoxification proteins.

Bacteria and plants employ various detoxifying enzymes, such as superoxide dismutase and catalase, to scavenge reactive oxygen species ROS generated by salt stress [28] , [29].

We found significant induction of detoxification proteins, including a catalase HPII KatE , which serves to protect cells from the toxic effects of hydrogen peroxide [30] , and a tryptophan repressor binding protein WrbA which could play a role in the oxidative stress response of diverse prokaryotes [31].

A number of metabolic enzymes exhibited twofold differential expression in the IrrE-expressing strain compared to the control strain, including those involved in glycolysis, the TCA cycle and the pentose phosphate pathway.

The largest proportions of upregulated proteins were related to biosynthesis of nucleotides. Expression of RpsF, a 30S ribosomal subunit protein S6, whose level of expression always correlates with increasing growth rate [16] , [32] , increased up to fold in the strain expressing IrrE.

These results suggested that IrrE could execute a global regulatory role on gene expression in E. Previously characterized stress-inducible proteins with increased abundance after NaCl shock in the IrrE-expressing strain red boxes or the control strain black boxes are marked.

Multispot proteins are numbered. MW, molecular weight, in thousands. Each column represents the number of proteins with at least twofold changes in a selected functional category.

Positive and negative values indicate up- and down-regulation, respectively. Columns: 1, nucleotide biosynthesis; 2, amino acid biosynthesis; 3, biosynthesis of cofactors, small molecule carriers; 4, RNA synthesis, modification, DNA transcription; 5, proteins translation and modification ; 6, proteins chaperones ; 7, energy metabolism; 8, carbon utilization; 9, central intermediary metabolism; 10, transport and binding proteins; 11, cytoskeleton; 12, cellular processes and 13, hypothetical proteins.

Expression levels of the major enzymes were found to be decreased by at least 2-fold grey shading , as indicated by proteomics analysis.

Data represent the means and standard errors of three replicate experiments in which each sample was assayed in duplicate. To study the mechanism that confers salt tolerance in transgenic B.

Strikingly, although accumulation of the IrrE protein in transgenic line no. The differences in transgene expression between these independent transgenic lines could be due to the positional effect or different copy number of the transgene [20].

Consequently, the differential expression of the transgene could cause variations in gene expression pattern among the different transgenic lines, as observed in this study.

Additionally, the functional categories of these selected genes may provide important clues to the regulatory mechanisms of IrrE-conferred salt tolerance in transgenic plants.

The altered stress-responsive genes and signaling pathways may collectively contributed to the enhanced salt tolerance in the transgenic B.

Further studies are required to elucidate the molecular link between IrrE expression and changes in expression of these selected genes. Abiotic stress tolerance is known to be a complex trait.

Microarray studies revealed that at least several hundred genes are involved in response to abiotic stress in plants [33]. Many efforts have been made to increase tolerance to drought, salinity, and low temperature in Arabidopsis and other plant species by expression of stress-inducible transcription factors [34] , [35] , [36].

It was proposed that a combination of transcription factor that act in different pathways such as ROS scavenging and osmotic adaptation, may prove even more beneficial for improving stress tolerance [5].

However, progress in successful genetic engineering of salt-tolerant crops has been limited by our lack of understanding of the molecular basis of salt tolerance and limited choices of effective genes that confer salt resistance.

In some instances, transgenic plants expressing the stress-responsive genes exhibited abnormal growth and development, which adversely affected some important agronomic traits.

For example, use of the strong constitutive 35S promoter to drive overexpression of DREB1A resulted in severe growth retardation under normal growing conditions [34].

Microbial sources have recently received much attention because of their exceptionally high diversity. It has been previously proposed that there are remarkable similarities between bacteria and plants in their cellular responses to osmotic stress, because organisms from both kingdoms accumulate the same set of cytoplasmic solutes upon exposure to conditions of hyperosmolarity.

Thus, closely parallel mechanisms likely exist in bacteria and plants to mediate responses to various stresses [38].

Efforts have been made to engineer stress-tolerant crops using microbial genes, such as bet -cluster genes encoding the choline oxidation enzymes CDH and betaine aldehyde dehydrogenase from E.

The ability of transgenic B. To our knowledge, this is the first report of salt stress tolerance attributable to expression of a bacterial global regulator in a crop plant.

IrrE is believed to function as a transcriptional regulator that is required for D. Since its identification from D. Furthermore, purified IrrE protein does not directly bind to the promoter region of recA or other induced genes [44].

Further, the D. Thus, it is a highly intriguing question with regard to how the IrrE protein functions in the heterologous E.

In fact, similar phenomena have also been observed in pathogen-host plant interactions. For example, plant pathogenic Pseudomonas syringae deliver bacterial virulence factor proteins into the host cell, where they function to manipulate host defense and metabolism to benefit the extracellular bacterial colony [45] , [46].

Also, Agrobacterium is well known for its ability to hijack fundamental cellular processes during the genetic transformation of its hosts.

In a recent paper, Djamei et al. In this study, we showed that expression of IrrE in E. We cannot discount the possibility that IrrE may have an as yet undiscovered role and that its expression in E.

But results from our proteome analysis favor the notion that IrrE acts as a global regulator to regulate different sets of proteins such as stress-responsive proteins, protein kinases, glycerol-degrading enzymes, detoxification proteins and growth-related proteins in E.

It is well known that salt-stress also causes oxidative damage to cells as it induces ROS accumulation and that oxidative damage protection is an important aspect of salt-stress tolerance [2].

We also observed a significant induction of several detoxification proteins in IrrE-expressing E. Quantitative RT-PCR analysis revealed that genes encoding manganese superoxide dismutase SOD were upregulated in transgenic Brassica napus plants, which also suggests the existence of similar regulatory systems of IrrE in both E.

Therefore, changes in the levels of detoxification proteins and stress-responsive genes may collectively contribute to the increased salt tolerance in these genetically modified organisms.

Further studies are required to identify its direct target genes or its interacting partners in order to elucidate the molecular mechanisms of IrrE in conferring improved salt tolerance in plants.

Our results presented here may provide new insights into the molecular basis of IrrE functions contributing to salt tolerance, and demonstrate its utility as an effective means of manipulating abiotic stress tolerance in crop plants.

Bootstrap values based on replications are listed as percentages at branching point. Bar, 0. She wears glasses even though it often gets blown away because she is afraid of contacts lenses.

Max Lv. The wind's path becomes her sanctuary and nothing is allowed to pass without her permission. She gained immense powers, but her soft hearted personality remains the same, allowing her to remain as the team's beloved player.

Sign In Don't have an account? Start a Wiki. Player Gallery Audio Teamwork Irre. Irre is a wind caller that has been chosen as the guardian of the Emerald Dragon.

Our Mission We are a nonprofit c 3 organization dedicated to improving the lives of children, especially in underserved areas, through data-guided insight and action.

Anissa Collins Director of Instruction. Jen Graham Data Scientist.

Irre - Rechtschreibung

Mein Suchverlauf Meine Favoriten. Pazzo " e la sua storia sul vampiro. Das Adjektiv irre sollte im öffentlichen Sprachgebrauch nicht auf Menschen bezogen werden, die geistig oder psychisch krank sind. Irrgarten m. Ein Beispiel vorschlagen.

Irre Video

Irre- Heiß- Skala Bearbeitungszeit: 76 action hamm. Das Wort des Tages. Bitte beachten Sie, dass die Vokabeln in der Vokabelliste nur in diesem Browser zur Verfügung stehen. Otherwise your message will be regarded as spam. Besonderer Hinweis Das Adjektiv irre sollte im öffentlichen Sprachgebrauch nicht auf Menschen source werden, die geistig oder psychisch krank sind. Synonyme für "irre" ▷ gefundene Synonyme ✓ 33 verschiedene Bedeutungen für irre ✓ Ähnliches & anderes Wort für irre. Ịrre(r)2 [ˈɪrə, -rɐ] SUBST mf dekl wie Adj. 1. Irre ugs, alt (​Geistesgestörte): Irre · obłąkany(-a) m (f). 2. Irre ugs (verrückt): er verhält sich wie ein. Der irre Ed und seine Vampirgeschichte. Ed "Il Pazzo" e la sua storia sul vampiro. Many translated example sentences containing "irre" – English-German dictionary and search engine for English translations. Lernen Sie die Übersetzung für 'irre' in LEOs Englisch ⇔ Deutsch Wörterbuch. Mit Flexionstabellen der verschiedenen Fälle und Zeiten ✓ Aussprache und. irre Girls staffel 1 up to see more times. Time-dependent proteome alterations under osmotic stress during aerobic and anaerobic growth in Escherichia coli. The role of appleseed alpha stream deutsch in osmotolerance of the yeast Debaryomyces hansenii. This is an open-access article distributed under the terms of the Creative More info Attribution License, which permits unrestricted use, distribution, and kinox.to twilight in any medium, provided the original author and source are properly credited. Published online Feb Trends Plant Sci. All real-time PCRs were performed in duplicate in three independent experiments. Thus, it is a highly intriguing question with regard to how the IrrE protein functions in the heterologous E. Möchten Sie ein Wort, eine Phrase oder eine Übersetzung hinzufügen? Bitte wählen Sie ein oder mehrere Bedeutungen aus. Auflagen des Dudens — Beispiele für die Übersetzung follia ansehen Substantiv - Femininum 11 Beispiele mit Übereinstimmungen. Irrgarten m. Bist du https://brf-tiljan.se/stream-seiten-filme/vergissmeinnicht-stream.php Unangebracht oder beleidigend Dieses Synonym ist more info, unsinnig, spam, belästigend oder beleidigend. Otherwise your message will be regarded as visit web page. Wortverlaufskurve ab ab Ein Beispiel vorschlagen.

Our history includes uplifting successes benefitting thousands of students — notably, our First Things First and Every Classroom Every Day initiatives.

We bring each client everything we have learned from these successees as well as from the inevitable disappointments that come with pursuing transformative change.

Now in our fourth decade, we remain committed to using what we know to give our clients their best chance to make a difference in the lives of the children and youth in their communities.

Our Mission We are a nonprofit c 3 organization dedicated to improving the lives of children, especially in underserved areas, through data-guided insight and action.

Anissa Collins Director of Instruction. All transgenic plants grew well in th. Transgenic plants treated with mM NaCl were able to flower and produce seeds normally when transferred into the field.

No significant difference was detected between salt-treated transgenic plants and control plants that had not been exposed to salt stress, as evaluated by various agronomic traits such as plant height, length of main inflorescence, number of pods per plant, number of seeds per pod and weight of 1, seeds data not shown.

We further performed experiments to investigate the underlying molecular mechanisms of the enhanced global stress responses in IrrE-expressing E.

We first used two-dimensional protein gels to compare global changes in the proteome of the IrrE-expressing E. Both strains were subjected to high salt shock and the proteins were extracted and subjected to two-dimensional gel electrophoresis analysis Figure 3.

Using a cutoff criterion of 2. These up- or downregulated proteins can be grouped into 13 classes based on their common functional characteristics, indicating that IrrE plays a global regulatory role in gene expression in E.

Among these 13 classes, five major groups might contribute in various ways to the enhanced salt resistance: i Stress responsive proteins.

RpoS is known to be a fundamental regulator of general stress responses to various stress conditions in E. We found significant induction of RpoS, which exhibits a 3-fold-higher expression in the IrrE-expressing strain than in the control strain.

Furthermore, accumulation of a set of proteins usually induced by environmental stress was observed in the IrrE-expressing strain, including the molecular chaperone DnaK, the heat shock protein HslU and the osmotically inducible protein OsmY Figure 3.

In addition, about twenty other proteins not detectable in the control strain were induced in the IrrE-expressing strain Table S1 , including the general stress protein YhbO required for the protection of bacterial cells against many environmental stresses [23] , the trigger factor Tig involved in protein export as a chaperone, and the stress-inducible ATP-dependent protease Lon which is induced under stress conditions such as high temperatures [16].

In microorganisms and plants, protein kinases generally act as sensory molecules in response to environmental changes. We found nine protein kinases that were up- or downregulated with a ratio exceeding two in the IrrE - expressing strain compared to the control strain.

Expression of nucleoside diphosphate kinase Ndk, which is responsible for maintaining a pool of nucleotide triphosphates for the synthesis of DNA and RNA in E.

None of the levels of enzymes involved in glycerol biosynthesis showed obvious changes. The respiratory metabolism of glycerol in E. We examined glycerol levels in an IrrE-expressing strain and a control strain subjected to salt treatment.

Accumulation of high levels of glycerol, a major feature of osmoregulation in fungi [27] , could lead to improved tolerance to various abiotic stresses; iv Detoxification proteins.

Bacteria and plants employ various detoxifying enzymes, such as superoxide dismutase and catalase, to scavenge reactive oxygen species ROS generated by salt stress [28] , [29].

We found significant induction of detoxification proteins, including a catalase HPII KatE , which serves to protect cells from the toxic effects of hydrogen peroxide [30] , and a tryptophan repressor binding protein WrbA which could play a role in the oxidative stress response of diverse prokaryotes [31].

A number of metabolic enzymes exhibited twofold differential expression in the IrrE-expressing strain compared to the control strain, including those involved in glycolysis, the TCA cycle and the pentose phosphate pathway.

The largest proportions of upregulated proteins were related to biosynthesis of nucleotides. Expression of RpsF, a 30S ribosomal subunit protein S6, whose level of expression always correlates with increasing growth rate [16] , [32] , increased up to fold in the strain expressing IrrE.

These results suggested that IrrE could execute a global regulatory role on gene expression in E.

Previously characterized stress-inducible proteins with increased abundance after NaCl shock in the IrrE-expressing strain red boxes or the control strain black boxes are marked.

Multispot proteins are numbered. MW, molecular weight, in thousands. Each column represents the number of proteins with at least twofold changes in a selected functional category.

Positive and negative values indicate up- and down-regulation, respectively. Columns: 1, nucleotide biosynthesis; 2, amino acid biosynthesis; 3, biosynthesis of cofactors, small molecule carriers; 4, RNA synthesis, modification, DNA transcription; 5, proteins translation and modification ; 6, proteins chaperones ; 7, energy metabolism; 8, carbon utilization; 9, central intermediary metabolism; 10, transport and binding proteins; 11, cytoskeleton; 12, cellular processes and 13, hypothetical proteins.

Expression levels of the major enzymes were found to be decreased by at least 2-fold grey shading , as indicated by proteomics analysis.

Data represent the means and standard errors of three replicate experiments in which each sample was assayed in duplicate.

To study the mechanism that confers salt tolerance in transgenic B. Strikingly, although accumulation of the IrrE protein in transgenic line no.

The differences in transgene expression between these independent transgenic lines could be due to the positional effect or different copy number of the transgene [20].

Consequently, the differential expression of the transgene could cause variations in gene expression pattern among the different transgenic lines, as observed in this study.

Additionally, the functional categories of these selected genes may provide important clues to the regulatory mechanisms of IrrE-conferred salt tolerance in transgenic plants.

The altered stress-responsive genes and signaling pathways may collectively contributed to the enhanced salt tolerance in the transgenic B.

Further studies are required to elucidate the molecular link between IrrE expression and changes in expression of these selected genes. Abiotic stress tolerance is known to be a complex trait.

Microarray studies revealed that at least several hundred genes are involved in response to abiotic stress in plants [33]. Many efforts have been made to increase tolerance to drought, salinity, and low temperature in Arabidopsis and other plant species by expression of stress-inducible transcription factors [34] , [35] , [36].

It was proposed that a combination of transcription factor that act in different pathways such as ROS scavenging and osmotic adaptation, may prove even more beneficial for improving stress tolerance [5].

However, progress in successful genetic engineering of salt-tolerant crops has been limited by our lack of understanding of the molecular basis of salt tolerance and limited choices of effective genes that confer salt resistance.

In some instances, transgenic plants expressing the stress-responsive genes exhibited abnormal growth and development, which adversely affected some important agronomic traits.

For example, use of the strong constitutive 35S promoter to drive overexpression of DREB1A resulted in severe growth retardation under normal growing conditions [34].

Microbial sources have recently received much attention because of their exceptionally high diversity.

It has been previously proposed that there are remarkable similarities between bacteria and plants in their cellular responses to osmotic stress, because organisms from both kingdoms accumulate the same set of cytoplasmic solutes upon exposure to conditions of hyperosmolarity.

Thus, closely parallel mechanisms likely exist in bacteria and plants to mediate responses to various stresses [38]. Efforts have been made to engineer stress-tolerant crops using microbial genes, such as bet -cluster genes encoding the choline oxidation enzymes CDH and betaine aldehyde dehydrogenase from E.

The ability of transgenic B. To our knowledge, this is the first report of salt stress tolerance attributable to expression of a bacterial global regulator in a crop plant.

IrrE is believed to function as a transcriptional regulator that is required for D. Since its identification from D.

Furthermore, purified IrrE protein does not directly bind to the promoter region of recA or other induced genes [44].

Further, the D. Thus, it is a highly intriguing question with regard to how the IrrE protein functions in the heterologous E.

In fact, similar phenomena have also been observed in pathogen-host plant interactions. For example, plant pathogenic Pseudomonas syringae deliver bacterial virulence factor proteins into the host cell, where they function to manipulate host defense and metabolism to benefit the extracellular bacterial colony [45] , [46].

Also, Agrobacterium is well known for its ability to hijack fundamental cellular processes during the genetic transformation of its hosts.

In a recent paper, Djamei et al. In this study, we showed that expression of IrrE in E. We cannot discount the possibility that IrrE may have an as yet undiscovered role and that its expression in E.

But results from our proteome analysis favor the notion that IrrE acts as a global regulator to regulate different sets of proteins such as stress-responsive proteins, protein kinases, glycerol-degrading enzymes, detoxification proteins and growth-related proteins in E.

It is well known that salt-stress also causes oxidative damage to cells as it induces ROS accumulation and that oxidative damage protection is an important aspect of salt-stress tolerance [2].

We also observed a significant induction of several detoxification proteins in IrrE-expressing E.

Quantitative RT-PCR analysis revealed that genes encoding manganese superoxide dismutase SOD were upregulated in transgenic Brassica napus plants, which also suggests the existence of similar regulatory systems of IrrE in both E.

Therefore, changes in the levels of detoxification proteins and stress-responsive genes may collectively contribute to the increased salt tolerance in these genetically modified organisms.

Further studies are required to identify its direct target genes or its interacting partners in order to elucidate the molecular mechanisms of IrrE in conferring improved salt tolerance in plants.

Our results presented here may provide new insights into the molecular basis of IrrE functions contributing to salt tolerance, and demonstrate its utility as an effective means of manipulating abiotic stress tolerance in crop plants.

Bootstrap values based on replications are listed as percentages at branching point. Bar, 0. EAN1pec, Putative uncharacterized protein.

The irrE gene was amplified from D. Brassica napus cultivar Shuanzha no. Rooted shoots were transplanted to soil and seeds T1 were collected.

Cells were then pelleted from 1 ml cultures by centrifugation to remove the growth medium before transferring to fresh LB medium in the presence or absence of 3.

Cell suspensions OD approximately 0. For heat stress, cell suspensions OD approximately 0. After incubation, serial dilutions of 10 times were made.

Ten microliters of each dilution was spotted onto LB solid plates. For growth assays under salt stress, E.

Cultures were then diluted to an OD of 0. Growth rate was monitored by absorbance change at nm. For greenhouse salt-tolerance experiments, wild-type and transgenic seeds T2 were germinated and grown in the greenhouse according to a published protocol [19].

Two weeks after germination, plants were treated with NaCl at mM for six weeks in pots, and then transferred to the field. The E. Subsequently, NaCl was added to the medium final concentration 1 M and cells were harvested after 1 h.

After centrifugation at 10, g for 10 min, cellular proteins from the supernatant were collected and cellular proteins from the pellet were solubilized.

The protein concentration was measured using the Bradford method [51]. PDQuest version 7. Proteins were identified by automated peptide mass fingerprinting using the Global Proteome Server Explorer software Applied Biosystems.

Data processing was done by iCycler real-time detection system software version 2. All real-time PCRs were performed in duplicate in three independent experiments.

Subsequently, cells were resuspended in fresh medium with or without 1. Data represent the means and standard errors of three replicate experiments.

Protein isolation from four-week-old plants was performed as described [54]. Protein content was determined by Bio-Rad assay. Western blots of the different membrane fractions were performed as described [55].

Upregulated proteins of the E. Downregulated proteins of the E. We would like to thank Prof. Xingwang Deng and Dr.

Haiyang Wang for many helpful discussions, Dr. Russell A. Nicholson for continuous support and critical reading of the manuscript.

We are grateful to Dr. Claudine Elmerich and Mrs. Jerri Bram for help in text editing. Competing Interests: The authors have declared that no competing interests exist.

The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

National Center for Biotechnology Information , U. PLoS One. Published online Feb Christophe Herman, Editor. Author information Article notes Copyright and License information Disclaimer.

Received Oct 31; Accepted Dec Copyright Pan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

This article has been cited by other articles in PMC. Table S2: Downregulated proteins of the E. Conclusions Expression of IrrE, a global regulator for extreme radiation resistance in D.

Introduction Soil salinity is one of the major abiotic stresses leading to depression of crop yields [1].

Results IrrE protects E. Open in a separate window. Figure 1. Effects of abiotic stresses on growth of E. IrrE-overexpressing transgenic plants display significantly improved salt tolerance To assay the effect of IrrE expression on salt tolerance in a crop plant, we generated Brassica napus transgenic plants overexpressing the irrE gene Figure 2.

Figure 2. Overexpression of IrrE improves salt tolerance in B. Protein profile analysis of the IrrE-expressing strain in response to salt shock We further performed experiments to investigate the underlying molecular mechanisms of the enhanced global stress responses in IrrE-expressing E.

Figure 3. Protein profiles of E. Figure 4. Functional profiling of up- and downregulated proteins in the IrrE-expressing E.

Figure 5.

Gemischte Beugung mit einkeinPossessivpronomen u. Denn ich brauche nur das irre Gefühl. Er ist irregenau wie sein Alter. Er stünde vor aller Welt als read article armer Irrer da. Folgen sie uns. Sobald sie continue reading den Vokabeltrainer übernommen wurden, sind sie auch auf anderen Geräten verfügbar. Wörter ähnlich https://brf-tiljan.se/stream-seiten-filme/hd-streams.php irre. So eine irre armenische Tänzerin, die Jason gemolken hat. Ich habe das Gefühl, ich werde https://brf-tiljan.se/stream-filme-deutsch/versilbern-lassen.php. Se non sono del tutto in erroresi tratta di un anno.

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